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                                                              FASLG Human
                                                              英文名稱(chēng):FASLG Human總訪問(wèn):293
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                                                              產(chǎn)地/品牌:prospec產(chǎn)品類(lèi)別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:CYT-051 最后更新:2024-12-5
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                                                              Catalogue Number CYT-051
                                                              Synonyms Fas ligand (TNF superfamily, member 6), APT1LG1, FASL, TNFSF6, CD178, tumor necrosis factor (ligand) superfamily member 6, Apoptosis antigen ligand, Fas antigen ligand, APTL, CD95-L.
                                                              Introduction The type II transmembrane protein FASLG is a member of the tumor necrosis factor (TNF) superfamily. A fas ligand/receptor interaction has a significant part in the regulation of the immune system and the advancement of cancer. FASLG is expressed on the activated T cell surface as a nondisulfidelinked homotrimer. FASLG binding to Fas/CD95/TNFRSF6 on a nearby cell prompts apoptosis in the Fas expressing cell. FASLG is released from the cell surface by metalloproteinases as a soluble molecule that stays trimeric and is able to bind with Fas, but its capability to activate apoptosis is radically reduced. In addition, FASLG binds to DcR3 - a soluble trap receptor with no signal transduction capabilities. Flawed Fas-mediated apoptosis causes oncogenesis in addition to drug resistance in existing tumors. Constitutive expression of FASLG in a variety of tumors enables their immune evasion. Both mouse and human FASLG are active on mouse and human cells.
                                                              Description Recombinant Human FAS Ligand produced in HEK293 cells is a non-glycosylated, polypeptide chain containing 147 amino acids (134-281a.a).
                                                              FASLG is fused to a 6 amino acid His-tag at N-terminus and purified by proprietary chromatographic techniques.
                                                              Source HEK293 cells.
                                                              Physical Appearance Sterile Filtered colorless solution.
                                                              Formulation

                                                              The FASLG solution (0.6mg/ml) contains 1xPBS.

                                                              Stability

                                                              FASLG Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C.
                                                              Please prevent freeze thaw cycles.

                                                              Purity Greater than 95.0% as determined by:
                                                              (a) Analysis by SEC-HPLC.
                                                              (b) Analysis by SDS-PAGE.
                                                              Biological Activity

                                                              Fas ligand is biologically active as determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The expected ED50< 10 ng/ml, corresponding to a specific activity of 1x105 units/mg.

                                                              Usage ProSpec''s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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