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                                                              HemC | porphobilinogen-Deaminase (PBG)
                                                              英文名稱:HemC | porphobilinogen-Deaminase (PBG)總訪問:305
                                                              國產(chǎn)/進口:進口半年訪問:5
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS08375 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

                                                              immunogen

                                                              overexpressed Thermosynechococcus elongatus HemC Q8DIE4

                                                              antibody format

                                                              rabbit

                                                              polyclonal

                                                              serum

                                                              lyophilized

                                                              quantity

                                                              200 µl

                                                              for reconstitution add 200 µl of sterile water.

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB)

                                                              related products

                                                              AS07 226 | anti-HemF

                                                              AS07 228 | anti-HemH | protoporphyrin ferrochelatase

                                                              additional information

                                                              to be added when available

                                                              application information
                                                              recommended dilution

                                                              1: 1000 - 1: 5000 (WB)

                                                              expected | apparent MW

                                                              34.7 kDa

                                                              confirmed reactivity

                                                              Thermosynechococcus elongatus

                                                              predicted reactivity

                                                              cyanobacteria

                                                              not reactive in

                                                              Synechococcus sp. PCC 6803

                                                              additional information

                                                              to be added when available

                                                              selected references

                                                              to be added when available


                                                              application example

                                                              0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

                                                              Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon

                                                              wesern blot detection using anti-PBG (HemC) antibodies
                                                              bio-equip.com
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